human ifn γ elispot pro kit alp  (Mabtech Inc)

Journal: Vaccines

Article Title: Cellular Immune Response and T Cell Epitope Mapping of Plasmodium falciparum Chimeric Vaccine Candidate GMZ2.6c and Its Components (MSP-3, GLURP and Pfs48/45) in Individuals Naturally Exposed to Malaria in Brazilian Amazon

doi: 10.3390/vaccines14050423

Figure Lengend Snippet: IFN-γ responses to MSP-3, GLURP, and Pfs48/45 synthetic peptides. Frequency of positive responders ( A ) and numbers of spots-forming units (SFU) ( B ) of IFN-γ responses to Mp 1 (blue), Ppp I (green), Ppp II (gray), and Gpp I (red) in exposed P. falciparum -infected (PFI, n = 20), non-infected (NI, n = 25), and non-endemic control (Control, n = 10) individuals. Bars represent the frequency of responders ( A ) and medians of adjusted SFU of IFN-γ responses to each peptide pool ( B ). Lines represent the interquartile ranges. Dashed red lines represent the positivity limit. Statistical significance was calculated using chi-square, Mann–Whitney test, and Kruskal–Wallis test, followed by Dunn’s post hoc test. Significant differences are indicated by * (* p < 0.05; *** p < 0.0005).

Article Snippet: ELISpot assays were carried out using the commercial kit ELISpot Plus: Human IFN-γ (ALP) (MabTech, Nacka Strand, Sweden) according to the manufacturer’s instructions.

Techniques: Infection, Control, MANN-WHITNEY

Journal: bioRxiv

Article Title: Duration of Initial Viremia Modulates Functional Properties of HIV-specific T Cell Receptors

doi: 10.64898/2026.01.29.702605

Figure Lengend Snippet: Breadth and magnitude of HIV-specific CTL responses in PBMCs, measured by IFN-g ELISPOT to five clade C HLA-B*58:01-restricted consensus epitopes (TW10, IW9, KW11, RY11, and HW9) in the 12 participants, measured during the acute phase of HIV-1 infection. Reported values represent spot-forming units (SFUs) after subtraction of background activity from triplicate negative control wells without peptide stimulation . Each column corresponds to an individual epitope, with response magnitudes depicted by a color scale. Numbers within each box indicate SFUs per 10 6 PBMCs. The intensity scale shown on the right denotes the strength of responses above background. Early and late treated persons are indicated by identifiers shown in red and blue boxes, respectively.

Article Snippet: IFN-gamma ELISPOT assays were performed according to the manufacturer’s instructions with the human IFN-gamma ELISPOT Basic Kit (ALP) (Cat. 3420-2 A, Mabtech).

Techniques: Enzyme-linked Immunospot, Infection, Activity Assay, Negative Control